Journal: Scientific Reports
Article Title: Reactive oxygen species–mediated switching expression of MMP-3 in stromal fibroblasts and cancer cells during prostate cancer progression
doi: 10.1038/s41598-017-08835-9
Figure Lengend Snippet: Detection of MMP-3 and -10 expression in clinical specimens of prostate cancer patients. Representative digital images of IHC staining of MMP-3 ( a ) and MMP-10 ( b ) in paired prostate tumor and normal prostate tissues from two individual patients in sections of a human prostate tissue microarray. Left, overview image of the tissue core; right, enlargement of the indicated area. The arrow and arrowhead respectively indicate positive staining in stromal and epithelial cells. Serum samples from benign prostatic hyperplasia (BPH) patients ( n = 12) and prostate cancer patients with a Gleason score ≤ 7 (low grade, n = 8) or ≥8 (high grade, n = 40) were tested for MMP-3 through an ELISA ( c ). Statistical analysis was performed using unpaired Student’s t -tests.
Article Snippet: A prostate adenocarcinoma tissue microarray was obtained from Super Bio Chips (CA4, Seoul, Korea), which contained 49 tissue cores representing samples from 40 cases of prostate cancer and nine matched normal adjacent tissues.
Techniques: Expressing, Immunohistochemistry, Microarray, Staining, Enzyme-linked Immunosorbent Assay